• Anna Schotthoefer PhD

  • Project Scientist I

    • Integrated Research and Development Laboratory
    • 1000 North Oak Ave. 2R3 Marshfield, WI 54449


2010 to 2012:
Integrated Research and Development Laboratory, Marshfield Clinic Research Institute, Marshfield
2007 to 2010:
Division of Vector-Borne Infectious Diseases, Centers for Disease Control and Prevention - Divison of Vector-Borne Infectious Diseases, Fort Collins
2003 to 2005:
Department of Pathobiology, Division of Parasitology, University of Illinois at Urbana-Champaign, Urbana
PhD, Department of Pathobiology, Division of Parasitology, University of Illinois at Urbana-Champaign, Urbana
MS, Biology Department, Wake Forest University, Winston-Salem
BS, Department of Biology, Cornell Univerisity, Ithaca

Work Experience

2011 to 2011:
Preceptor, Masters of Public Health Program, University of Wisconsin School of Medicine and Public Health, Madison
2006 to 2010:
Adjunct Faculty and Instructor, Department of Biology, Metropolitan State College of Denver, Denver
2005 to 2006:
Visiting Assistant Professor, Department of Pathobiology, University of Illinois at Urbana-Champaign, Urbana
1994 to 1995:
Lab Technician Senior, Virginia Institute of Marine Science, College of William & Mary, Gloucester Point
1993 to 1994:
Lab Technician, Haskin Shellfish Research Laboratory, Rutgers University, Port Norris


Dr. Schotthoefer's research focuses on the epidemiology, ecology and clinical manifestations of tick-borne diseases, including Lyme disease, anaplasmosis, and babesiosis. She has interests in better understanding the interactions between environmental, genetic, and human behavioral risk factors associated with the diseases in Wisconsin, and in improving the clinical diagnosis and treatment of the diseases. She has helped develop molecular tools that may be used for more accurate and timely clinical diagnoses of the diseases and for learning more about the genetic strains of the pathogens that are found in the Midwest. Current efforts are aimed at analyzing data captured in the electronic health record to improve our understanding of the clinical symptoms and outcomes associated with the diseases and to define better guidelines for recognizing and treating the diseases.

Research Interests

epidemiology and ecology of tick-borne dieases in Wisconsin; applying analytical methods to clinical data in the electronic health record to improve diagnosis and treatment of tick-borne diseases; genetic analysis of disease pathogens

Select Publications

  • Girard YA, Travinsky B, Schotthoefer AM, Fedorova N, Eisen RJ, Eisen L,...Lane RS. (2009). Population structure of the Lyme borreliosis spirochete Borrelia burgdorferi in the western black-legged tick (Ixodes pacificus) in northern California. Appl Environ Microbiol. 75(22):7243-7252.
    PubMed ID: 19783741
  • Schotthoefer AM, Meece JK, Ivacic LC, Bertz PD, Zhang KQi, Weiler T,...Fritsche TR [including Uphoff TS.] (2013 July). Comparison of a real-time PCR Method with serology and blood smear analysis for diagnosis of human anaplasmosis:importance of Infection time course for optimal test utilization. JOURNAL OF CLINICAL MICROBIOLOGY. 51(7):2147-53.
    PubMed ID: 23637292
  • Schotthoefer AM, Meece JK, Fritsche TR. (2014 June). A clinical, diagnostic, and ecologic perspective on human anaplasmosis in the Upper Midwest. WMJ. 113(3):107-14; quiz 115.
    PubMed ID: 25118439
  • Rohr JR, Civitello DJ, Crumrine PW, Halstead N, Miller AD, Schotthoefer AM,...Beasley VR. (2015 March). Predator diversity, intraguild predation, and indirect effects drive parasite transmission. Proc Natl Acad Sci U S A.. 112(10):3008-13.
    PubMed ID: 25713379
  • Schotthoefer AM, Frost HM. (2015 December). Ecology and Epidemiology of Lyme Borreliosis. Clin Lab Med. 35(4):723-43.
    PubMed ID: 26593254
  • Levin AE, Williamson PC, Bloch EM, Clifford J, Cyrus S, Shaz BH,...Busch MP [including Schotthoefer AM, Fritsche TR.] (2016 May 25). Serologic screening of United States blood donors for Babesia microti using an investigational enzyme immunoassay. Transfusion.
    PubMed ID: 27224258
  • Mareedu N, Schotthoefer AM, Tompkins J, Hall MC, Fritsche TR, Frost HM. (2017 July 10). Risk Factors for Severe Infection, Hospitalization, and Prolonged Antimicrobial Therapy in Patients with Babesiosis. Am J Trop Med Hyg.
    PubMed ID: 28722598
  • Schotthoefer AM, Hall MC, Vittala S, Bajwa R, Frost HM. (2017 May 16). Clinical Presentation and Outcomes of Children With Human Granulocytic Anaplasmosis. J Pediatric Infect Dis Soc.
    PubMed ID: 28520981
  • Snyder JL, Giese H, Bandoski-Gralinski C, Townsend J, Jacobson BE, Shivers R,...Lowery TJ [including Schotthoefer AM, Fritsche TR, Green C.] (2017 August). T2 Magnetic Resonance Assay-Based Direct Detection of Three Lyme Disease-Related Borrelia Species in Whole-Blood Samples. J Clin Microbiol. 55(8):2453-2461.
    PubMed ID: 28566314
  • Schotthoefer AM, Schrodi SJ, Meece JK, Fritsche TR, Shukla SK. (2017). Pro-inflammatory immune responses are associated with clinical signs and symptoms of human anaplasmosis. PLoS One. 12(6):e0179655.
    PubMed ID: 28628633