• Patrick Allaire Ph.D.

  • Project Scientist I


    • MCRI - MCRI-Precision Med Research
    • 1000 North Oak Ave. ML8 Marshfield, WI 54449
    • (715) 389-3146

Education

2017 to Present:
Genetics and Medical Bioinformatics, Marshfield Clinic Research Institute, Marshfield, Wisconsin
2011 to 2017:
Genetics and Molecular/Cell Biology, University of Utah, Salt Lake City, Utah
2009 to 2011:
Molecular/Cellular Neuroscience, Montreal Neurological Institute - McGill University, Montreal, Canada
2003 to 2009:
Ph.D., Molecular/Cellular Neuroscience, Montreal Neurological Institute - McGill University, Montreal, Canada
2000 to 2002:
M.Sc., Experimental Medicine - Oncology, McGill University, Montreal, Canada
1997 to 2000:
B.Sc., Biochemistry, University of Montreal, Montreal, Canada

Work Experience

2017 to 2019:
Post Doctoral Fellow, Mashfield Clinic Research Instute, Marshfield
2011 to 2017:
Post Doctoral Fellow, University of Utah, Salt lake City
2009 to 2011:
Post doctoral Fellow, Montreal Neurological Institute, Montreal

Honors & Awards

2009 to 2009:
C. Nance Common Neuroscience Award
2013 to 2015:
American Heart Association Postdoctoral Award
2018 to Present:
MCRI Internal Grant Award

Statement

Genetic research using model organisms is used as a springboard to study pathways responsible for organ development and function. This has greatly guided and benefited human health research. With the rapid growth of DNA sequencing technologies and the growing importance of human genome research, we are making significant leaps in health improvement though disease prevention, diagnosis and prognosis. My goal is to be part of this exciting movement and to help integrate genetic knowledge to improve human health.

Research Interests

cancer development and endocrine malfunction
intracellular trafficking
genomic, proteomic and metabolomic data science

Professional Societies

2006 to 2010:
American Society for Cell Biology, member - participant
2013 to 2016:
American Heart Association, member - participant
2011 to 2018:
Genetic Society of America, member - participant

Presentations

2005:
Connecdenn, an AP-2-Binding Protein Linking DENN Domains to Clathrin-Mediated Trafficking in the Synapse (Oral), Annual meeting for Cell Biology, San Francisco
2007:
Connecdenn, a Novel Clathrin-coated Vesicle Associated Protein Linking DENN Domains to Endocytic Vesicle Trafficking (poster) , Annual meeting for Cell Biology, Washington
2008:
Function of the DENN Domain Revealed through Characterization of Connecdenn (poster), Annual meeting for Cell Biology, San Francisco
2009:
The Connecdenn 1 DENN Domain: A GEF for Rab35 Mediating Cargo-Specific Exit from Early Endosomes (poster, Annual meeting for Cell Biology, San Diego
2010:
Rab35 Regulates Recycling of Arf6 Cargo Required for Intercellular Adhesion and Differentiation (poster), Annual meeting for Cell Biology, Philadelphia
2011:
A Rab35/Arf6 recycling complex regulates cell adhesion and migration: implications for cancer biology (poster), Annual meeting for Cell Biology , San Francisco
2014:
CAB-1 is required for dense core vesicle trafficking and peptide hormone secretion (poster), Gordon Research Conference, New London, NH,“Protein Trafficking, Processing and Secretion”
2015:
CAB-1 has dual functions in dense core vesicle trafficking (poster), Gordon Research Conference, Andover, NH,“Molecular Membrane Biology”
2016:
CAB-1 has dual functions in dense core vesicle trafficking (poster), Gordon Research Conference, New London, NH,“Protein Trafficking, Processing and Secretion”

Select Publications

  • Levesque L, Lam MH, Allaire P, Mondat M, Houle S, Beaudoin G,...Leclerc G. (2001 Apr). Effects of radiation therapy on vascular responsiveness. J. Cardiovasc. Pharmacol.. 37(4):381-93.
    PubMed ID: 11300651
  • Blondeau F, Ritter B, Allaire PD, Wasiak S, Girard M, Hussain NK,...McPherson PS. (2004 Mar 16). Tandem MS analysis of brain clathrin-coated vesicles reveals their critical involvement in synaptic vesicle recycling. Proc. Natl. Acad. Sci. U.S.A.. 101(11):3833-8.
    PubMed ID: 15007177
  • Girard M, Allaire PD, McPherson PS, Blondeau F. (2005 Aug). Non-stoichiometric relationship between clathrin heavy and light chains revealed by quantitative comparative proteomics of clathrin-coated vesicles from brain and liver. Mol. Cell Proteomics. 4(8):1145-54.
    PubMed ID: 15933375
  • Girard M, Allaire PD, Blondeau F, McPherson PS. (2005 Apr). Isolation of clathrin-coated vesicles by differential and density gradient centrifugation. Curr Protoc Cell Biol. Chapter 3 :Unit 3.13.
    PubMed ID: 18228473
  • Allaire PD, Ritter B, Thomas S, Burman JL, Denisov AY, Legendre-Guillemin V,...McPherson PS. (2006 Dec 20). Connecdenn, a novel DENN domain-containing protein of neuronal clathrin-coated vesicles functioning in synaptic vesicle endocytosis. J. Neurosci.. 26(51):13202-12.
    PubMed ID: 17182770
  • Ritter B, Denisov AY, Philie J, Allaire PD, Legendre-Guillemin V, Zylbergold P,...McPherson PS. (2007 Sep 19). The NECAP PHear domain increases clathrin accessory protein binding potential. EMBO J.. 26(18):4066-77.
    PubMed ID: 17762867
  • Allaire PD, Marat AL, Dall'Armi C, Di Paolo G, McPherson PS, Ritter B. (2010 Feb 12). The Connecdenn DENN domain: a GEF for Rab35 mediating cargo-specific exit from early endosomes. Mol. Cell. 37(3):370-82.
    PubMed ID: 20159556
  • Allaire PD, Seyed Sadr M, Chaineau M, Seyed Sadr E, Konefal S, Fotouhi M,...McPherson PS. (2013 Feb 1). Interplay between Rab35 and Arf6 controls cargo recycling to coordinate cell adhesion and migration. J. Cell. Sci.. 126(Pt 3):722-31.
    PubMed ID: 23264734
  • Allaire PD, McPherson PS, Ritter B. (2015). Analysis of connecdenn 1-3 (DENN1A-C) GEF activity for Rab35. Methods Mol. Biol.. 1298 :217-31.
    PubMed ID: 25800846
  • Yerukala Sathipati S, Tsai MJ, Carter T, Allaire P, Shukla SK, Beheshti A, Ho SY. Survival estimation in patients with stomach and esophageal carcinoma using miRNA expression profiles. Computational and Structural Biotechnology Journal. 2022 August 20;20(2022):4490-4500. doi: https://doi.org/10.1016/j.csbj.2022.08.025. PMCID: PMC9421182.
    PubMed ID: 36051876
  • Allaire P, He J, Mayer J, Moat L, Gerstenberger P, Wilhorn R,...Hebbring S. Genetic and clinical determinants of telomere length. HGG Adv. 2023 April;4(3):100201. doi: 10.1016/j.xhgg.2023.100201. PMCID: PMC10199259.
    PubMed ID: 37216007